Study of the phenolic composition and antioxidant activity of the adaptogenic agent “Adaptofit” in vitro and in vivo
AUTHORS : Lubsandorzhievaone P.B. | Baldandorzhievaone M.V. | Shantanovaone L.N. | Osadchuk2 L.V.
RELEVANT UNIVERSITIES : oneFSBSI Institute of General and Experimental Biology SB RAS, Ulan-Ude, 2FGBUN Institute cytology and genetics SB RAS, Novosibirsk
YEAR : 2014
SUMMARY The quantitative content of the main groups of biologically active substances and the antioxidant activity in vitro and in vivo of the 15-component adaptogenic agent “Adaptofit” were determined. For in vitro experiments, the ethyl acetate fraction, decoction and dry extract from
Adaptophyt were used. In the dry extract from “Adaptofit”, the HPLC method established the content of the following phenolic compounds: gallic (31.7%), chlorogenic (21.8%), isoferulic (8%), chicoric (7.4%) acids, vitexin (3, 3%), routine (2.6%). The data of experiments in vitro showed that the most
effective Fe2 + – chelating activity is the dry extract, superoxide radical-binding activity – ethyl acetate fraction and dry extract, NO-binding activity – ethyl acetate fraction. Moreover, the ethyl acetate fraction most effectively suppresses the formation of malondialdehyde in the model with
lipoproteins than a dry extract or a decoction of Adaptofit
